Selection and optimization of the method for DNA isolation and purification from Corylus species for PCR analysis

Aim.The use of molecular genetic analysis tools requires DNA preparations high purity. However, in the case hazelnut plants (Corylus spp.), obtaining quality is complicated by presence a significant amount secondary metabolites leaf tissue. The aim study was to select and optimize method for isolation purification further PCR analysis. Methods. Three methods using cetavlon as detergent were used, which differ steps: commonly used protocol Doyle, 1987; modified with silicon dioxide microparticles; extraction soluble metabolites, precedes step. Results. first two shown be unsuitable from dry leaves due impurities. last allowed remove part water-soluble organic substances at stage obtain high-quality preparations. Conclusions. Corylus spp. hybrids selected optimized, allows sufficient dried tissue, suitable PCR.